Ethylenediaminetetraacetic acid (EDTA) wakili ne na ƙarfe. Abin da ake kira chelating yana nufin yana iya haɗawa da wasu ions na ƙarfe kuma ya sa ions ɗin ƙarfe da aka daure su daina aiki.
Yin amfani da wannan sifa ta EDTA, akwai nau'ikan aikace-aikace guda biyu a cikin gwaje-gwaje;
Nau'i ɗaya shine aikace-aikacen juyawa, wanda ke hana wasu halayen ta hanyar kutse tare da ɗaurin ions na ƙarfe.
Ɗauki ion: A wasu halayen, daurin ions na ƙarfe yana da mahimmanci. Mun haɗa EDTA tare da waɗannan ions na ƙarfe don hana wannan halayen.
Misali, coagulation jini yana buƙatar sa hannu na ions calcium. Muna ƙara EDTA zuwa jini don chelate calcium ions tare da EDTA, don haka hana jini coagulation. Wannan shine ka'idar EDTA anticoagulation;
Ga wani misali, shirye-shiryen warwarewa a cikin tabo mai cutarwa kuma yana amfani da tasirin chelation na EDTA. Mun san cewa idan ba a rage yawan ƙwayar calcium mai girma ba, zai yi wuya a yanke shi zuwa cikakkiyar nama lokacin yanka, don haka muna buƙatar yankewa kafin a yanka.
Hakanan akwai nau'in aikace-aikacen gaba wanda ke haɓaka wasu halayen ta hanyar tsoma baki tare da ɗaure ions ƙarfe.
Misali, ƙara EDTA zuwa maganin narkewar trypsin. Idan ba a ƙara EDTA ba, trypsin zai ɗaure da calcium da magnesium a saman tantanin halitta ko a cikin al'ada, don haka yana hana ayyukan trypsin.
A wannan lokacin, ƙara ɗan ƙaramin EDTA don ƙyale EDTA ta haɗu tare da waɗancan alli, magnesium da sauran ions masu ɗimbin yawa. A wannan lokacin, an halicci yanayi mai kyau don abin da ya faru na trypsin.
Abu mafi ban mamaki game da EDTA shine solubility. Yana da ɗan narkewa cikin ruwa kuma ya bayyana fari a cikin ruwa. A wannan lokacin, ƙara sodium hydroxide don daidaita ƙimar pH. Yayin da pH ke karuwa kuma mafi girma, maganin ya zama mai haske da haske. Lokacin da pH ke kusa da 8, ya zama cikakke. Ba ya tsoron acid amma alkali.
EDTA yana da amfani masu ban mamaki da yawa. Ana iya ganin cewa abubuwan da ke cikin yanayi suna da sihiri sosai, amma ba mu lura da shi ba.
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Lokacin aikawa: Mayu-13-2024