Ethylenediaminetetraacetic acid (EDTA) wani sinadari ne na ƙarfe mai hana ƙwai. Abin da ake kira chelating yana nufin cewa zai iya haɗuwa da wasu ions na ƙarfe kuma ya sa ions na ƙarfe da aka ɗaure ba su aiki.

Ta amfani da wannan halayyar EDTA, akwai nau'ikan aikace-aikace guda biyu a cikin gwaje-gwaje;

Nau'i ɗaya shine aikace-aikacen baya, wanda ke hana wasu halayen ta hanyar tsoma baki ga ɗaurewar ions na ƙarfe.

Ɗauki ion: A wasu halayen, ɗaure ion na ƙarfe yana da mahimmanci. Muna haɗa EDTA da waɗannan ion na ƙarfe don hana wannan amsawar.

Misali, hada jini yana buƙatar haɗin ions na calcium. Muna ƙara EDTA a cikin jini don cire ions na calcium tare da EDTA, don haka hana hada jini. Wannan shine ƙa'idar hana hada jini ta EDTA;

Ga wani misali, shirya maganin cire sinadarin calcium a cikin tabo na cututtuka shi ma yana amfani da tasirin chelation na EDTA. Mun san cewa idan ba a cire sinadarin calcium mai yawa ba, zai yi wuya a yanke shi gaba ɗaya yayin yanka shi, don haka muna buƙatar cire sinadarin calcium kafin yanke shi.

Akwai kuma wani nau'in aikace-aikacen gaba wanda ke haɓaka wasu halayen ta hanyar tsoma baki ga ɗaure ions na ƙarfe.

Misali, a ƙara EDTA a cikin maganin narkewar trypsin. Idan ba a ƙara EDTA ba, trypsin zai manne da calcium da magnesium a saman tantanin halitta ko a cikin hanyar al'ada, ta haka yana hana aikin trypsin.

A wannan lokacin, ƙara ɗan EDTA kaɗan don ba EDTA damar haɗuwa da waɗannan ƙwayoyin calcium kyauta, magnesium da sauran ions na divalent. A wannan lokacin, an ƙirƙiri yanayi mai kyau don faruwar trypsin.

Abu mafi ban mamaki game da EDTA shine yadda yake narkewa. Yana narkewa kaɗan a cikin ruwa kuma yana bayyana fari a cikin ruwa. A wannan lokacin, ƙara sodium hydroxide don daidaita ƙimar pH ɗinsa. Yayin da pH ke ƙaruwa da girma, maganin yana ƙaruwa. Lokacin da pH ɗin ya kusa da 8, yana bayyana gaba ɗaya. Ba ya jin tsoron acid amma alkaline.

EDTA tana da amfani mai yawa. Ana iya ganin cewa abubuwan da ke cikin yanayi suna da sihiri sosai, amma ba mu lura da su ba.

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